saa app ki mice  (Jackson Laboratory)

Journal: Scientific Reports

Article Title: Longitudinal characterization reveals behavioral impairments in aged APP knock in mouse models

doi: 10.1038/s41598-025-89051-8

Figure Lengend Snippet: APP-KI mice do not show progressive cognitive impairment through 12 months of age. Male and female APP SAA ( a , c , e , g ) and APP WT ( b , d , f , h ) mice were tested longitudinally from 4 through 12 month (m) of age, at the indicated time points across a battery of cognitive behavior tests. ( a , b ) Exploration ratio in the object location test (OLT). ( c , d) Percent alternation in the Y-maze. ( e , f ) Barnes maze performance during longitudinal training period and ( g , h ) 72 h probe trial. ( i , j ) Tris soluble and Triton soluble hippocampal Aß analyzed by multiplex ELISA. ( i ) Total Aβ including Aβ38, Aβ40, and Aβ42 ( j ) Aβ 42:40 ratio. A significant genotype by solubility interaction effect was observed in Aβ 42:40 ratio ( p = 0.0297). Points represent individuals (excluding ( e , f ) where points represent the group mean), bars represent the mean ± SEM. n = 10–11 APP WT and 13 APP SAA for ( a-h ) and n = 4 for ( i-j ). Data analyzed by one sample t test compared to 0.5 chance level for ( a , b ) repeated measures ANOVA and Dunnett’s multiple comparisons test to compare each age to 4 m timepoint ( c-h ) and 2-way ANOVA with Fisher’s LSD post-hoc tests I-J. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001.

Article Snippet: Female and male APP SAA KI (B6.Cg-Apptm1.1Dnli/J Strain #:034711) and APP WT KI (B6.Cg-Appem1Adiuj/J Strain #:033013) mice were acquired from Jackson Labs through a kind gift by Dr. Srikant Rangaraju (Emory University) and maintained as homozygotes.

Techniques: Battery, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Solubility

Journal: Scientific Reports

Article Title: Longitudinal characterization reveals behavioral impairments in aged APP knock in mouse models

doi: 10.1038/s41598-025-89051-8

Figure Lengend Snippet: APP SAA but not APP WT mice show spatial learning and memory deficits at 16 months of age. Young (2–3-month-old) and 16-month-old male and female APP SAA and APP WT mice were tested cross sectionally on a range of cognitive tests. ( a ) Exploration ratio in the object location test (OLT). ( b ) Percent alternation in the Y-maze. ( c-e ) Barnes maze performance during longitudinal training period showing both ( c ) primary latency and ( d ) area under the curve (AUC). ( e ) 72 h probe trial primary latency. Points represent individuals (excluding c , where points represent the group mean), bars represent the mean and SEM. n = 9–12 APP WT and 9–13 APP SAA . Data analyzed by one sample t test compared to 0.5 chance level for ( a ), 2-way ANOVA with Fisher’s LSD post hoc test for ( b , d and e ). * p ≤ 0.05; ** p ≤ 0.01.

Article Snippet: Female and male APP SAA KI (B6.Cg-Apptm1.1Dnli/J Strain #:034711) and APP WT KI (B6.Cg-Appem1Adiuj/J Strain #:033013) mice were acquired from Jackson Labs through a kind gift by Dr. Srikant Rangaraju (Emory University) and maintained as homozygotes.

Techniques: